The pathogen and symptomatology of pink disase of pineapple fruit in the Philippines. 62:446-450. yield of the former is poor. Pink disease is a major problem in the pineapple canning industry. A glucose dehydrogenase (Gdh) encoded by the gdhA gene has been implicated in the colour formation activity of P. citrea. The distribution of Acetobacter strains in Indonesia is discussed in light of isolation sources. Nutri, bacterial strains from Hawaii. These isolates exhibited typical pathogenicity reactions in pineapple juice tests, pineapple slices and fruit. Pink disease of pineapple is characterized by the production of distinct dark orange-brown color produced in the fruit tissue after the heating process of canning ().Since the fruits remain superficially symptomless in the field, eliminating the disease by culling fruits either in the field or at postharvest is highly problematic and therefore of economic importance. Calcium 2-keto-L-gulonate (Ca-2-KLG, a key intermediate in vitamin C synthesis) is produced from calcium 2,5-diketo D-gluconate (Ca-2,5-DKG) by a variety of bacteria. The pink disease of pineapple represents one of the most perplexing problems of the pineapple canned-fruit industry. 0000013867 00000 n Paenibacillus validus (formerly B. gordonae) has been tested against P. citrea for the biocontrol of pink disease, giving promising results, presumably (Cha et al., 1994; ... Plant diseases cause major yields decrease so it would be desirable to find alternatives for their control. gladioli UAPS07070 hampers its use as a biocontrol agent of pink disease . Our bacterial collection included species that possess the genes for production of Ca-2-KLG from Ca-2,5-DKG; however, the, 16S rRNA gene (rDNA) sequences from Acetobacter europaeus, Acetobacter xylinum, Acetobacter hansenii, Acetobacter liquefaciens, Acetobacter diazotrophicus, Acetobacter aceti, Acetobacter pasteurianus and Gluconobacter oxydans were determined. 0000002505 00000 n protein. The next thing I buy will be one of Del Monte’s pink pineapples! biogenesis of c-type cytochromes, which are known for their role as specific electron acceptors linked to dehydrogenase activities. The heating process required for canning Both G. oxydans strains 303D and 180 required biotin, nicotinic acid, and pantothenic acid for growth; E. herbicola 189 required only nicotinic acid; however, A aceti 295 was able to grow without any added supplements in glucose mineral salts medium. H‰b```f``¹ÅÀÆÀÀ÷AX¢,=*‚«¿ˆÐ°M``˜|í - pink disease of pineapple safe to eat - MedlinePlus: Diverticulosis and Diverticulitis. Bacteria were isolated from diseased locules of cotton bolls collected in a field in SC, USA and tested for the ability to cause comparable disease symptoms in greenhouse grown cotton fruit. Pineapples are susceptible to a disease called pink disease, Pink Pineapple Disease If you have ever eaten canned pineapple, chances are you’ve seen or eaten a piece of pineapple with a reddish or pink hue. Phylogenetic trees were constructed that reflected the distant and close relationships. 0000013018 00000 n 0000006592 00000 n 0000007027 00000 n In this paper it has been shown that P. citrea contains a second, homologous gdh gene and its product, GdhB, represents the main source of Gdh activity in this organism. Pathogenic isolates were categorized as Pantoea agglomerans on the basis of phenotype testing, fatty acid profiling (similarity index = 0.94), and 16s ribosomal DNA sequence analysis (99% nucleotide identity). trailer << /Size 67 /Info 24 0 R /Root 26 0 R /Prev 200636 /ID[<5accc8dfc7f255738cec48ff6a1e6098><5accc8dfc7f255738cec48ff6a1e6098>] >> startxref 0 %%EOF 26 0 obj << /Type /Catalog /Pages 23 0 R >> endobj 65 0 obj << /S 153 /Filter /FlateDecode /Length 66 0 R >> stream In fruit cocktail, it’s easy to assume cherry juice stained the pineapple, but that might not be the case at all. Antisera against whole cells of Gluconobacter oxydans and, Forty-six strains of acetic acid bacteria newly isolated from flowers, fruits, and fermented foods collected in Indonesia were taxonomically studied. Pantoea agglomerans isolates from field-collected immature, diseased cotton caused comparable infection symptoms in greenhouse produced cotton fruit. Several pineapple cultivars are also resistant to certain disease and the disease resistance can be additive or quantitative. Elsewhere, pineapple heart rot disease is reported to be devastating with the incidence of 25-45% (Shen et al., 2013;Shreenivasa et al., 2015) and associated yield loss of … ; The pathogen responsible for causing pink disease remained obscure and the nature of the pink color formation of the pineapple fruit tissue was not understood. To investigate the aetiology of seed and boll rot of cotton grown in South Carolina (SC). If you have ever eaten canned pineapple, chances are you’ve seen or eaten a piece of pineapple with a reddish or pink hue. Pantoea citrea is a Gram-negative, facultative anaerobic, non-spore forming, bacilliform bacterium with physiological and biochemical as well as 16S rDNA features corresponding to those of the Enterobacteriaceae. 0000013197 00000 n Pantoea citrea, the causal agent of pink disease of pineapple, harbors a cryptic plasmid of 5229 bp. These are some of the most common pineapple problems and some hints for dealing with pineapple problems: Rohrbach, K. G., and Pfeiffer, J. ; The pathogen responsible for causing pink disease remained obscure and the nature of the pink color formation of the pineapple fruit tissue was not understood. 0000002693 00000 n The sequence of the entire genome is forthcoming and should, shed a complete picture on the organization of operons and genes, disease pathogen from flower to flower. A procedure for the preparation of spheroplasts in Ca-2,5-DKG- and Ca-2-KLG-producing bacteria was developed for the construction of recombinants (fusants), combining the genes for conversion of glucose to Ca-2-KLG efficiently by protoplast fusion. nov. for Group XI. The mealybugs are generally found on the surface of pineapples, but can also be found inside the closed blossom cups (Jahn 1995). No members of the Acetobacteriaceae are known to be plant pathogens, thus neither G. oxydans nor A. aceti have been previously reported as specific plant pathogens. The pathogen and, 10. 25 0 obj << /Linearized 1 /O 27 /H [ 1242 322 ] /L 201264 /E 24013 /N 5 /T 200646 >> endobj xref 25 42 0000000016 00000 n Molecular genetic approaches were applied to elucidate t … Cho, J. J., Hayward, A. C., and Rohrbach, K. G. 1980. Still, pineapple has health benefits you should consider before you cut it out of your diet. They were Gram-negative rods, produced acetic acid from ethanol, oxidized acetate and lactate to CO(2) and H(2)O, and had Q-9 as the major ubiquinone system. To … symptomatology of pink disase of pineapple fruit in the Philippines. Van, 5. Pink disease represents one, pathogen responsible for causing pink disease remained obscure and, not understood. 4. Manual spray application of Bacillus gordniae 2061R inoculum covering five 25 acre-replicates during optimum flowering period. `š xجĕkæŽɸ˜,t¸â%PØ. Primavera (PRI) and the cv. Some pests and diseases cause cosmetic damage that while not reducing yield, results in a complete loss of the fruit economic value. 0000004740 00000 n All investigated species had unique 16S rDNA sequences by, Indirect immunofluorescence tests with antisera against whole cells of Phenylobacterium immobile strains revealed a serological relationship to Pseudomonas vesicularis, Aquaspirillum itersonii and Rhodospirillum rubrum, three members of the purple nonsulfur bacteria (group I) and also to Gluconobacter oxydans and Azotobacter vinelandii. Bacteria which cause pink disease of pineapple, identified on the basis of their nutritional and biochemical activities, were found to belong to three genera. ABSTRACT-Pink disease is an asymptomatic pineapple disease in the field and is evidenced with a red-dark coloration when the infected fruit is processed to obtain products such as juice, jam, and preservatives. In the last few years, Pantoea citrea has been described as the causative agent of pink disease. By sea and land, Pinkglow™ arrives from afar and into your hands. Rec. The major problems faced by pineapple growers worldwide are mealybug wilt and the associated mealybugs, ants, and virus; soil-borne pests including root-knot and reniform nematodes, symphylids, and Phytophthora; and fungi attacking the fruit such as fusariosis. There, diketogluconate by 2-ketogluconate dehydr, presumably due to the condensation and the formation, dark red reaction is shown in Fig. pink disease of pineapple is cause by bacterium acetobacter aceti Blushed, because it is saying that the pineapple did a human action, in blushing. colonies are entire, smooth, glistening, translucent, but not mucoid. Pink disease is a major problem in the pineapple canning industry. All figure content in this area was uploaded by Clarence I Kado, All content in this area was uploaded by Clarence I Kado on Feb 05, 2015, (L.) Merr.] detection prior to canning. © 2008-2020 ResearchGate GmbH. are therefore economically important in the production of fresh and canned fruit products. Tatumella ptyseos, an Unrevealed Causative Agent of Pink Disease in Pineapple, Antagonistic interactions among bacteria inhabiting pineapple, Genomics of Pineapple Disease-Resistance Genes, Role of Pantoea agglomerans in opportunistic bacterial seed and boll rot of cotton (Gossypium hirsutum) grown in the field, gdhB, a gene encoding a second quinoprotein glucose dehydrogenase in Pantoea citrea, is required for pink disease of pineapple, Genetic and Biochemical Characterization of the Pathway in Pantoea citrea Leading to Pink Disease of Pineapple, The Interaction of Four Bacteria Causing Pink Disease of Pineapple with Several Pineapple Cultivars, Nutritional requirements and biochemical activities of pineapple pink disease bacterial strains from Hawaii, Characterization of pUCD5000 Involved in Pink Disease Color Formation byPantoea citrea. Besides the genetic makeup that causes the pink disease reaction in the pineapple fruit, the bacterium elicits the hypersensitive response in tobacco (2). The identification and classification of NBS genes in pineapple provide a valuable genomic resource and improve the understanding of pineapple R genes, which further facilitates the development of pineapple disease-resistant cultivars. No members of the, heterogeneous collection of yellow and semi-yellow pigm, Using molecular genetics approaches, the causal agent of pink, biochemical pathway leading to the pink coloration associated with, and fruit can be attributed to the ability of, biochemical pathway illustrated in Fig. Affected fruit acquire a brownish pigment after pasteurization and can contaminate non-affected fruit before they are released to the consumer. No isolates were identified as A. aceti (Group I), A. peroxydans (Group III), and A. estunensis (Group VI). Attem, member of the alpha-proteobacteria) and En, member of the beta-proteobacteria). What Exactly Is a Pink Pineapple? 0000004634 00000 n The disease is virtually asymptomatic in the field. Example of an immature pineapple fruit bearing blossoms that are commonly visited by flying insects. Pantoea citrea, a member of the family Enterobacteriaceae, causes pink disease of pineapple, whose symptom is characterized by the formation of pink to brown discolorations of the infected portions of the pineapple fruit cylinder upon canning. It is due to absorption of mercury . Pink disease of pineapple, caused by Pantoea citrea, is characterized by a dark coloration on fruit slices after autoclaving. This coloration is initiated by the oxidation of glucose to gluconate, which is followed by further oxidation of gluconate to as yet unknown chromogenic compounds. Pink disease is of little importance in fresh fruit, but can be a very serious sporadic problem in processed fruit because of the lack of. demonstrated high sequence similarities (99.6 to 94.2%, corresponding to 7 to 83 base differences). to the fruit, the high correlation of higher, Copyright 2003 by The American Phytopathological Society. Over 250 isolates showed a very high similarity in their phenotypic and genotypic traits with Tatumella ptyseos, a close relative of Pantoea. Anton. When cultural practices fail to provide sufficient control, many different insecticides, miticides, nematicides, and fungicides are used to protect the crop from damage.